MCM3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33028

Novus Biologicals, part of Bio-Techne
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NBP1-33028
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MCM3 Antibodies »

Product Summary for MCM3 Antibody

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human MCM3. The exact sequence is proprietary.

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MCM3 Antibody

Western Blot: MCM3 Antibody [NBP1-33028]

Western Blot: MCM3 Antibody [NBP1-33028]

Western Blot: MCM3 Antibody [NBP1-33028] - Sample (30 ug of whole cell lysate) A: 293T 7.5% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: MCM3 Antibody [NBP1-33028]

Immunocytochemistry/ Immunofluorescence: MCM3 Antibody [NBP1-33028]

Immunocytochemistry/Immunofluorescence: MCM3 Antibody [NBP1-33028] - Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MCM3 protein stained by MCM3 antibody, C-term diluted at 1:500. Blue: Hoechst 33343 staining.
Immunohistochemistry-Paraffin: MCM3 Antibody [NBP1-33028]

Immunohistochemistry-Paraffin: MCM3 Antibody [NBP1-33028]

Immunohistochemistry-Paraffin: MCM3 Antibody [NBP1-33028] - Paraffin-embedded NCIN87 Xenograft, using antibody at 1:100 dilution.

Applications for MCM3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MCM3

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.

Alternate Names

cervical cancer proto-oncogene 5, DNA polymerase alpha holoenzyme-associated protein P1, DNA replication factor MCM3, DNA replication licensing factor MCM3, EC 3.6.4.12, HCC5, hRlf beta subunit, MCM3 minichromosome maintenance deficient 3, MCM3 minichromosome maintenance deficient 3 (S. cerevisiae), MGC1157, minichromosome maintenance complex component 3, minichromosome maintenance deficient (S. cerevisiae) 3, minichromosome maintenance deficient 3, P1.h, p102, P1-MCM3, replication licensing factor, beta subunit, RLF subunit beta, RLFB

Gene Symbol

MCM3

Additional MCM3 Products

Product Documents for MCM3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MCM3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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