Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all MCM7 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 300 and 350 of human Minichromosome Maintenance 7 using the numbering given in entry NP_005907.3 (GeneID 4176).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for MCM7 Antibody - BSA Free

Western Blot: MCM7 Antibody [NB100-252]

Western Blot: MCM7 Antibody [NB100-252]

Western Blot: MCM7 Antibody [NB100-252] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-MCM7 antibody used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: MCM7 Antibody [NB100-252]

Western Blot: MCM7 Antibody [NB100-252]

Western Blot: MCM7 Antibody [NB100-252] - Whole cell lysate (60 ug) from HeLa cells. Antibody used at 0.25 ug/ml.

Applications for MCM7 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MCM7

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]

Long Name

Minichromosome Maintenance Protein 7

Alternate Names

CDC47, MCM2, P1CDC47, P85MCM, PNAS146, PPP1R104, Protein Phosphatase 1

Entrez Gene IDs

4176 (Human)

Gene Symbol

MCM7

UniProt

P33993

Additional MCM7 Products

Product Documents for MCM7 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MCM7 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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