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MCT1/SLC16A1 Antibody (14612)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43838

Novus Biologicals, part of Bio-Techne
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NBP2-43838

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 14612

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human MCT1/SLC16A1. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MCT1/SLC16A1 Antibody (14612)

Western Blot: MCT1/SLC16A1 Antibody (14612) [NBP2-43838]

Western Blot: MCT1/SLC16A1 Antibody (14612) [NBP2-43838]

Western Blot: MCT1/SLC16A1 Antibody (14612) [NBP2-43838] - Unboiled various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MCT1 antibody [14612] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Immunocytochemistry/ Immunofluorescence: MCT1/SLC16A1 Antibody (14612) [NBP2-43838]

Immunocytochemistry/ Immunofluorescence: MCT1/SLC16A1 Antibody (14612) [NBP2-43838]

Immunocytochemistry/Immunofluorescence: MCT1/SLC16A1 Antibody (14612) [NBP2-43838] - Analysis of Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MCT1 protein stained by MCT1 antibody [14612] diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: MCT1/SLC16A1 Antibody (14612) [NBP2-43838]

Immunohistochemistry-Paraffin: MCT1/SLC16A1 Antibody (14612) [NBP2-43838]

Immunohistochemistry-Paraffin: MCT1/SLC16A1 Antibody (14612) [NBP2-43838] - Human colon. MCT1 stained by MCT1 antibody [14612] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Applications for MCT1/SLC16A1 Antibody (14612)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS, 20% Glycerol

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MCT1/SLC16A1

The SLC16A1 gene encodes a monocarboxylate transporter (MCT1) that mediates the movement of lactate and pyruvate across cell membranes Import and export of these substrates by tissues such as erythrocytes, muscle, intestine, and kidney are ascribed largely to the action of a proton-coupled MCT (Garcia et al., 1994 [PubMed 8124722]).[supplied by OMIM]

Long Name

Monocarboxylic Acid Transporter 1 (Solute Carrier Family 16 Member 1)

Alternate Names

HHF7, SLC16A1

Gene Symbol

SLC16A1

Additional MCT1/SLC16A1 Products

Product Documents for MCT1/SLC16A1 Antibody (14612)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MCT1/SLC16A1 Antibody (14612)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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