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MCT1/SLC16A1 Antibody (9D12)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15184

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15184

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9D12

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthesized peptide derived from human MCT1/SLC16A1. UniProt ID (P53985).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MCT1/SLC16A1 Antibody (9D12)

Western Blot: MCT1/SLC16A1 Antibody (9D12) [NBP3-15184]

Western Blot: MCT1/SLC16A1 Antibody (9D12) [NBP3-15184]

Western Blot: MCT1/SLC16A1 Antibody (9D12) [NBP3-15184] - Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, 293T whole cell lysate, K562 whole cell lysate. All lanes: MCT1/SLC16A1 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 54, 47 KDa. Observed band size: 54 KDa.
Immunohistochemistry: MCT1/SLC16A1 Antibody (9D12) [NBP3-15184]

Immunohistochemistry: MCT1/SLC16A1 Antibody (9D12) [NBP3-15184]

Immunohistochemistry: MCT1/SLC16A1 Antibody (9D12) [NBP3-15184] - IHC image of MCT1/SLC16A1 antibody diluted at 1:100 and staining in paraffin-embedded human colon cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunohistochemistry: MCT1/SLC16A1 Antibody (9D12) [NBP3-15184]

Immunohistochemistry: MCT1/SLC16A1 Antibody (9D12) [NBP3-15184]

Immunohistochemistry: MCT1/SLC16A1 Antibody (9D12) [NBP3-15184] - IHC image of MCT1/SLC16A1 antibody diluted at 1:100 and staining in paraffin-embedded human breast cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Applications for MCT1/SLC16A1 Antibody (9D12)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 150mM NaCl, and 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: MCT1/SLC16A1

The SLC16A1 gene encodes a monocarboxylate transporter (MCT1) that mediates the movement of lactate and pyruvate across cell membranes Import and export of these substrates by tissues such as erythrocytes, muscle, intestine, and kidney are ascribed largely to the action of a proton-coupled MCT (Garcia et al., 1994 [PubMed 8124722]).[supplied by OMIM]

Long Name

Monocarboxylic Acid Transporter 1 (Solute Carrier Family 16 Member 1)

Alternate Names

HHF7, SLC16A1

Gene Symbol

SLC16A1

Additional MCT1/SLC16A1 Products

Product Documents for MCT1/SLC16A1 Antibody (9D12)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MCT1/SLC16A1 Antibody (9D12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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