Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1A2E3

Format

BSA Free

Concentration

1 mg/ml

View all MD2 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a recombinant protein corresponding to amino acids 2 to 160 of human MD-2.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for MD-2 Antibody (1A2E3) - BSA Free

Western Blot: MD-2 Antibody (1A2E3)BSA Free [NBP1-75513]

Western Blot: MD-2 Antibody (1A2E3)BSA Free [NBP1-75513]

Western Blot: MD-2 Antibody (1A2E3) [NBP1-75513] - Analysis of (A) 25 and (B) 125 ng of MD-2 recombinant protein with MD-2 antibody at 1 ug/ml.
MD-2 Antibody (1A2E3) - BSA Free

Immunocytochemistry/ Immunofluorescence: MD-2 Antibody (1A2E3) - BSA Free [NBP1-75513] -

Immunocytochemistry/ Immunofluorescence: MD-2 Antibody (1A2E3) - BSA Free [NBP1-75513] - Immunofluorescence of MD-2 in human spleen with MD-2 Antibody at 20 u/ml.

Applications for MD-2 Antibody (1A2E3) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

2 ug/ml

Immunohistochemistry-Paraffin

2 ug/ml

Western Blot

1 ug/ml

Reviewed Applications

Read 1 review rated 5 using NBP1-75513 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 degrees C. Avoid freeze/thaw cycles.

Background: MD2

MD-2 is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs). TLRs are signaling molecules that recognize different pathogen-associated molecular patterns (PAMPs) and serve as an important link between the innate and adaptive immune responses. TLR4, the major signaling receptor for lipopolysaccharide (LPS), requires the binding of MD-2 to its extracellular region for maximal response to LPS. The specificity of this response is determined by the species of MD-2; e.g., human MD-2 transfected into mouse cells can cause mouse TLR4 to react to LPS analogs that are normally antagonistic to human but not mouse TLR4.

Long Name

Myeloid Differentiation Protein 2/Lymphocyte Antigen 96

Alternate Names

ESOP-1, LY96, MD-2

Gene Symbol

LY96

UniProt

NP_056179 (Human)

Additional MD2 Products

Product Documents for MD-2 Antibody (1A2E3) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MD-2 Antibody (1A2E3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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