MD-2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-56655
Key Product Details
Validated by
Orthogonal Validation, Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A full length recombinant protein was used as the immunogen.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for MD-2 Antibody
Western Blot: MD-2 Antibody [NB100-56655]
Western Blot: MD-2 Antibody [NB100-56655] - Lanes containing cell lysate from untreated cell are on the left (-) and lysates from IFNG treated cells are on the right (+). The proteins are identified by the corresponding gene names. The housekeeping enzyme glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was blotted as a control that is not altered by IFNG treatment. Except for GAPDH, the numbers under each gene name are the fold changes in mRNAs. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0185956) licensed under a CC-BY license. anti-TLR4 (Novus Biological NBP2-24538).Western Blot: MD-2 Antibody [NB100-56655]
Western Blot: MD-2 Antibody [NB100-56655] - Inactivation of MEK-ERK1/2 and NF-kappaB signalling rescues the adverse effects of MD-1 deficiency on Ang II-induced myocyte hypertrophy. Representative western blot of quantitative analysis of the TLR4 and MD-2 protein levels in WT and MD-1-/- mice at 4 weeks after sham or AB surgery (n = 4). *P < 0.05 vs. WT/shams; #P < 0.05 vs. WT/AB. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep41857) licensed under a CC-BY license.Immunohistochemistry-Paraffin: MD-2 Antibody [NB100-56655]
Immunohistochemistry-Paraffin: MD-2 Antibody [NB100-56655] - Analysis of MD2 in human brain tissue using an isotype control antibody (top left) and MD2 antibody (bottom left, right) at 10 ug/ml.Applications for MD-2 Antibody
Application
Recommended Usage
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
5-10 ug/ml
Western Blot
0.5-2 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MD2
Long Name
Myeloid Differentiation Protein 2/Lymphocyte Antigen 96
Alternate Names
ESOP-1, LY96, MD-2
Gene Symbol
LY96
UniProt
Additional MD2 Products
Product Documents for MD-2 Antibody
Product Specific Notices for MD-2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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