MD-2 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77201
Conjugate
Catalog #
Key Product Details
Validated by
Tagged Protein Expression
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
ELISA, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Antibody was raised against a peptide corresponding to 13 amino acids near the center of human MD-2. The immunogen is located within the last 50 amino acids of MD-2. Amino Acid Sequence: CSFKGIKFSKGKYK
Specificity
Human MD-2 has 2 isoforms, including isoform 1 (160aa, 18kD) and isoform 2 (130aa, 15kD). Mouse MD-2 has one isoform (160aa, 18kD) and Rat MD-2 also has one isoform (160aa, 18kD). NBP1-77201 can detect human, mouse and rat.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for MD-2 Antibody - BSA Free
Western Blot: MD-2 AntibodyBSA Free [NBP1-77201]
Western Blot: MD-2 Antibody [NBP1-77201] - Western Blot Validation in Rat Tissue Lysates. Loading: 15 ug of lysates per lane.Antibodies: MD-2, NBP1-77201 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Western Blot: MD-2 AntibodyBSA Free [NBP1-77201]
Western Blot: MD-2 Antibody [NBP1-77201] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human MD-2 recombinant protein per lane. Antibodies: MD-2, NBP1-77201 (Lane 1: 0.5 ug/mL, Lane 2: 1 ug/mL and Lane 3: 2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Western Blot: MD-2 AntibodyBSA Free [NBP1-77201]
Western Blot: MD-2 Antibody [NBP1-77201] - Western Blot Validation in Daudi Cell Lysate. Loading: 15 ug of lysates per lane.Antibodies: MD-2, NBP1-77201 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Applications for MD-2 Antibody - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
10-20 ug/mL
Immunohistochemistry
2-2.5 ug/mL
Immunohistochemistry-Paraffin
2 ug/ml
Western Blot
0.5-1 ug/mL
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MD2
Long Name
Myeloid Differentiation Protein 2/Lymphocyte Antigen 96
Alternate Names
ESOP-1, LY96, MD-2
Gene Symbol
LY96
UniProt
Additional MD2 Products
Product Documents for MD-2 Antibody - BSA Free
Product Specific Notices for MD-2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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