Key Product Details

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI8C10

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all IFIH1/MDA5 Antibodies »

Product Summary for MDA5 Antibody (OTI8C10) - Azide and BSA Free

Immunogen

Full length human recombinant protein of human IFIH1 (NP_071451) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

116.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MDA5 Antibody (OTI8C10) - Azide and BSA Free

Western Blot: MDA5 Antibody (OTI8C10)Azide and BSA Free [NBP2-72604]

Western Blot: MDA5 Antibody (OTI8C10)Azide and BSA Free [NBP2-72604]

Western Blot: MDA5 Antibody (OTI8C10) - Azide and BSA Free [NBP2-72604] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MDA5.

Applications for MDA5 Antibody (OTI8C10) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IFIH1/MDA5

Gene ID: 64135 DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein that is upregulated in response to treatment with beta-interferon (IFNB) and a protein kinase C-activating compound, mezerein (MEZ). Irreversible reprogramming of melanomas can be achieved by treatment with both these agents; treatment with either agent alone only achieves reversible differentiation.

Long Name

Melanoma Differentiation-Associated Protein 5

Alternate Names

AGS7, Helicard, Hlcd, IDDM19, IFIH1, IMD95, MDA-5, RH116, SGMRT1

Gene Symbol

IFIH1

Additional IFIH1/MDA5 Products

Product Documents for MDA5 Antibody (OTI8C10) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MDA5 Antibody (OTI8C10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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