MDA5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB110-1244

Novus Biologicals, part of Bio-Techne
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NB110-1244
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Primate

Cited:

Human, Mouse

Applications

Validated:

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all IFIH1/MDA5 Antibodies »

Product Specifications

Immunogen

Peptide with sequence SNGYSTDENFRYL-C corresponding to N-Terminus according to NP_071451.2.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27237091).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for MDA5 Antibody

Immunohistochemistry-Paraffin: MDA5 Antibody [NB110-1244]

Immunohistochemistry-Paraffin: MDA5 Antibody [NB110-1244]

Immunohistochemistry-Paraffin: MDA5 Antibody [NB110-1244] - Staining in paraffin embedded Human Pancreas. Antibody at 10 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
Flow Cytometry: MDA5 Antibody [NB110-1244]

Flow Cytometry: MDA5 Antibody [NB110-1244]

Flow Cytometry: MDA5 Antibody [NB110-1244] - Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for MDA5 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunohistochemistry

10 ug/mL

Immunohistochemistry-Paraffin

10 ug/mL

Peptide ELISA

Detection limit 1:32000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IFIH1/MDA5

Gene ID: 64135 DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein that is upregulated in response to treatment with beta-interferon (IFNB) and a protein kinase C-activating compound, mezerein (MEZ). Irreversible reprogramming of melanomas can be achieved by treatment with both these agents; treatment with either agent alone only achieves reversible differentiation.

Long Name

Melanoma Differentiation-Associated Protein 5

Alternate Names

AGS7, Helicard, Hlcd, IDDM19, IFIH1, IMD95, MDA-5, RH116, SGMRT1

Entrez Gene IDs

64135 (Human)

Gene Symbol

IFIH1

UniProt

NP_071451. (Human)

Additional IFIH1/MDA5 Products

Product Documents for MDA5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MDA5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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