Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all IFIH1/MDA5 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 16 amino acid synthetic peptide from near the center of human MDA5. The immunogen is located within amino acids 330 - 380 of MDA5. Amino Acid Squence: AKDHLDKKKKASEPGK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MDA5 Antibody - BSA Free

Western Blot: MDA5 AntibodyBSA Free [NBP1-76761]

Western Blot: MDA5 AntibodyBSA Free [NBP1-76761]

Western Blot: MDA5 Antibody [NBP1-76761] - Daudi cell lysate with MDA5 antibody at (A) 1, (B) 2 and (C) 4 ug/mL.
Immunocytochemistry/ Immunofluorescence: MDA5 Antibody - BSA Free [NBP1-76761]

Immunocytochemistry/ Immunofluorescence: MDA5 Antibody - BSA Free [NBP1-76761]

Immunocytochemistry/Immunofluorescence: MDA5 Antibody [NBP1-76761] - Immunofluorescence of MDA5 in Human Lymph Node cells with MDA5 antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: MDA5 Antibody - BSA Free [NBP1-76761]

Immunohistochemistry-Paraffin: MDA5 Antibody - BSA Free [NBP1-76761]

Immunohistochemistry-Paraffin: MDA5 Antibody [NBP1-76761] - Human lymph node tissue with MDA5 antibody at 5 ug/ml.
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Applications for MDA5 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1-4 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IFIH1/MDA5

The innate immune system detects viral infection by recognizing various viral components and triggers antiviral responses. Like the toll-like receptor 3 (TLR3), the melanoma differentiation-associated protein 5 (MDA5) recognizes double-stranded (ds) RNA, a molecular pattern associated with viral infection. MDA5, a member of the DEAD/DEAH-box RNA helicase family, consists of an amino-terminal caspase recruitment domain (CARD) and a carboxyl-terminal RNA helicase domain similar to that of the related protein RIG-1. When stimulated by dsRNA, MDA5 recruits the adaptor protein VISA and ultimately causes the activation of IRF-3 and NF-kappaB. MDA5 and RIG-1 recognize different types of dsRNA, with MDA5 recognizing poly (I:C). MDA5-null mice were highly susceptible to infection with picornaviruses, which possess such sequences, demonstrating the importance of MDA5 in innate immunity.

Long Name

Melanoma Differentiation-Associated Protein 5

Alternate Names

AGS7, Helicard, Hlcd, IDDM19, IFIH1, IMD95, MDA-5, RH116, SGMRT1

Gene Symbol

IFIH1

UniProt

NP_071451 (Human)

Additional IFIH1/MDA5 Products

Product Documents for MDA5 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MDA5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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