MDC1 Antibody (P2B11) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-12890
![Western Blot: MDC1 Antibody (P2B11) [NBP2-12890] Western Blot: MDC1 Antibody (P2B11) [NBP2-12890]](https://resources.bio-techne.com/images/products/MDC1-Antibody-P2B11-Western-Blot-NBP2-12890-img0004.jpg)
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Bovine, Non-human Primate
Cited:
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # P2B11
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
GST-tagged recombinant protein corresponding to mouse MDC1 at and around the N-terminus
Localization
Nucleus
Specificity
Detects approx 184kDa. This antibody recognizes MDC1 at and around the N-terminus.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for MDC1 Antibody (P2B11) - BSA Free
Western Blot: MDC1 Antibody (P2B11) [NBP2-12890]
Western Blot: MDC1 Antibody (P2B11) [NBP2-12890] - Western Blot analysis of Mouse Cortex and Cerebellum showing detection of 184 kDa MDC1 protein using Mouse Anti-MDC1 Monoclonal Antibody, Clone P2B11 (NBP2-12890). Lane 1: MW ladder. Lane 2: Mouse Cortex. Lane 3: Mouse Cerebellum. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-MDC1 Monoclonal Antibody (NBP2-12890) at 1:1000 for 2 hours RT. Secondary Antibody: Goat Anti-Mouse at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 184 kDa.Immunocytochemistry/ Immunofluorescence: MDC1 Antibody (P2B11) [NBP2-12890]
Immunocytochemistry/Immunofluorescence: MDC1 Antibody (P2B11) [NBP2-12890] - Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-MDC1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 60X.Western Blot: MDC1 Antibody (P2B11) [NBP2-12890]
Western Blot: MDC1 Antibody (P2B11) [NBP2-12890] - analysis of Human 293Trap cell lysates showing detection of 184 kDa MDC1 protein using Mouse Anti-MDC1 Monoclonal Antibody, Clone P2B11 . Lane 1: MW ladder. Lane 2: 293Trap cell lysates. Load: 30 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-MDC1 Monoclonal Antibody at 1:1000 for 2 hours RT. Secondary Antibody: Goat Anti-Mouse HRP:IgG at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 184 kDa.Applications for MDC1 Antibody (P2B11) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:2000
Application Notes
0.5 ug/ml of MDC1 Antibody was sufficient for detection of MDC1 in 10 ug of HeLa cell lysate by ECL immunoblot analysis.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 50% Glycerol
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MDC1
Long Name
Mediator of DNA-damage checkpoint 1
Alternate Names
NFBD1
Gene Symbol
MDC1
Additional MDC1 Products
Product Documents for MDC1 Antibody (P2B11) - BSA Free
Product Specific Notices for MDC1 Antibody (P2B11) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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