MDH1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77780
![Western Blot: MDH1 Antibody [NBP1-77780]](https://resources.bio-techne.com/images/products/MDH1-Antibody-Western-Blot-NBP1-77780-img0001.jpg "Western Blot: MDH1 Antibody [NBP1-77780]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Porcine
Applications
ELISA, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
MDH1 [Pig Heart] (Uniprot: P11708)
Reactivity Notes
Cross reactivity against MDH1 from other tissues and species may occur but have not been specifically determined.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Description
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified Malate Dehydrogenase [Pig Heart]
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for MDH1 Antibody
Western Blot: MDH1 Antibody [NBP1-77780]
Western Blot: MDH1 Antibody [NBP1-77780] - Malate Dehydrogenase Antibody
MDH1 Antibody
Western Blot of Sheep anti-Malate Dehydrogenase antibody. Lane 1: Malate Dehydrogenase. Lane 2: None. Load: 50 ng per lane. Primary antibody: Malate Dehydrogenase antibody at 1:1,000 for overnight at 4C. Secondary antibody: Peroxidase sheep secondary antibody at 1:40,000 for 30 min at RT. Block for 30 min at RT. Predicted/Observed size: 36 kDa, 36 kDa for Malate Dehydrogenase. Other band(s): NoneApplications for MDH1 Antibody
Application
Recommended Usage
ELISA
1:5000-1:20000
Immunoprecipitation
1:100
Western Blot
1:500-1:5000
Application Notes
This product has been tested by western blot and is suitable to be assayed against 1.0 ug of Malate Dehydrogenase [Pig Heart] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:40,000 of the reconstitution concentration is suggested for this product.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent)
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: MDH1
Long Name
malate dehydrogenase 1
Alternate Names
DEE88, EIEE88, HEL-S-32, KAR, MDH-s, MDHA, MGC:1375, MOR2
Gene Symbol
MDH1
UniProt
Additional MDH1 Products
Product Documents for MDH1 Antibody
Product Specific Notices for MDH1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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