MDM2/HDM2 Antibody (HDM2-323) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB600-1312
![Western Blot: MDM2/HDM2 Antibody (HDM2-323)BSA Free [NB600-1312]](https://resources.bio-techne.com/images/products/MDM2-HDM2-Antibody-HDM2-323-Western-Blot-NB600-1312-img0003.jpg "Western Blot: MDM2/HDM2 Antibody (HDM2-323)BSA Free [NB600-1312]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # HDM2-323
Format
BSA Free
Concentration
2.0 mg/ml
Product Specifications
Immunogen
Synthetic peptide conjugated to KLH, corresponding to amino acids 389-402 of Human MDM2.
Epitope
The epitope recognized by the antibody is localized in the C-terminal region of MDM2 (amino acids 389-402 of human MDM2).
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Localization
Nuclear and cytoplasmic. Expressed predominantly in the nucleoplasm. Interaction with ARF (P14) results in the localisation of both proteins to the nucleus. The nucleolar localisation signals in both ARF and MDM2 may be necessary to allow efficient nucleo
Specificity
MDM2 (HDM2-323)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for MDM2/HDM2 Antibody (HDM2-323) - BSA Free
Western Blot: MDM2/HDM2 Antibody (HDM2-323)BSA Free [NB600-1312]
Western Blot: MDM2/HDM2 Antibody (HDM2-323) [NB600-1312] - Whole extracts of HEK-293 cells overexpressing human (lane 1) or mouse (lane 2) MDM2 were separated on SDS-PAGE and probed with 2 ug/mL Monoclonal Anti-MDM2 Clone: HDM2-323. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate.Applications for MDM2/HDM2 Antibody (HDM2-323) - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Western Blot
1-2 ug/ml
Application Notes
WB: Detects a band of approximately 90 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS (pH 7.4)
Format
BSA Free
Preservative
0.9% Sodium Azide
Concentration
2.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MDM2/HDM2
Long Name
Double Minute 2 Protein
Alternate Names
HDM2
Gene Symbol
MDM2
Additional MDM2/HDM2 Products
Product Documents for MDM2/HDM2 Antibody (HDM2-323) - BSA Free
Product Specific Notices for MDM2/HDM2 Antibody (HDM2-323) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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