MDM2/HDM2 Antibody (MDM2/7190R) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21092

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # MDM2/7190R

Format

Azide and BSA Free

Concentration

1 mg/ml

View all MDM2/HDM2 Antibodies »

Product Specifications

Immunogen

Recombinant human MDM2/HDM2 protein fragment (exact sequence is proprietary)

Localization

Nucleus.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

Positive Controls: Human liposarcoma.

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.

Scientific Data Images for MDM2/HDM2 Antibody (MDM2/7190R) - Azide and BSA Free

MDM2/HDM2 Antibody (MDM2/7190R) - Azide and BSA Free

MDM2/HDM2 Antibody (MDM2/7190R)

Formalin-fixed, paraffin-embedded human liposarcoma stained with MDM2/HDM2 antibody (MDM2/7190R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
MDM2/HDM2 Antibody (MDM2/7190R) - Azide and BSA Free

MDM2/HDM2 Antibody (MDM2/7190R)

Formalin-fixed, paraffin-embedded human bladder stained with MDM2/HDM2 antibody (MDM2/7190R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Applications for MDM2/HDM2 Antibody (MDM2/7190R) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: MDM2/HDM2

MDM2 is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues.

Long Name

Double Minute 2 Protein

Alternate Names

HDM2

Gene Symbol

MDM2

Additional MDM2/HDM2 Products

Product Documents for MDM2/HDM2 Antibody (MDM2/7190R) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MDM2/HDM2 Antibody (MDM2/7190R) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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