MDM2/HDM2 [p Ser185] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1683
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse
Applications
ELISA, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This affinity purified MDM2/HDM2 [p Ser185] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 175-200 of mouse MDM2/HDM2/HDM2. (Uniprot: P23804)
Reactivity Notes
A BLAST analysis was used to suggest minimal cross reactivity with MDM2/HDM2 homologues from other sources.
Modification
p Ser185
Localization
Nuclear and cytoplasmic. Expressed predominantly in the nucleoplasm. Interaction with ARF (P14) results in the localisation of both proteins to the nucleus. The nucleolar localisation signals in both ARF and MDM2 may be necessary to allow efficient nucleo
Specificity
This affinity-purified antibody is directed against the phosphorylated form of mouse MDM2/HDM2 protein at the pS185 residue. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against Mouse MDM2/HDM2 pS185 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated mouse MDM2/HDM2 is minimal by ELISA and western blot. A BLAST analysis was used to suggest minimal cross reactivity with MDM2/HDM2 homologues from other sources.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This affinity-purified antibody is directed against the phosphorylated form of mouse MDM2 protein at the p Ser185 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for MDM2/HDM2 [p Ser185] Antibody
Western Blot: MDM2/HDM2 [p Ser185] Antibody [NB100-1683] - Shown in a 293T whole cell lysate. Cells were serum-starved for 24 hours prior to harvest. Approximately 20 ug of lysate was loaded per lane for SDS-PAGE. Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/ml) for 20 min prior to harvest. Follow reaction of antibody with a 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.
![MDM2/HDM2 [p Ser185] Antibody](https://resources.bio-techne.com/images/products/nb100-1683_rabbit-polyclonal-mdm2-hdm2-p-ser185-antibody-2552023131318.jpg "MDM2/HDM2 [p Ser185] Antibody")
MDM2/HDM2 [p Ser185] Antibody
Affinity Purified Anti-MDM2 pS185 (Rabbit) is shown to detect a 102 kDa band (arrow) corresponding to phosphorylated mouse MDM2 present in a 293T whole cell lysate. Cells were serum-starved for 24 hours prior to harvest. Approximately 20 ug of lysate was loaded per lane for SDS-PAGE. Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/ml) for 20 min prior to harvest. Follow reaction of antibody with a 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.Applications for MDM2/HDM2 [p Ser185] Antibody
Application
Recommended Usage
ELISA
1:3000-1:12000
Immunoprecipitation
1:100
Western Blot
1:500-1:2000
Application Notes
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect bands approximately 102 kDa in size corresponding to phosphorylated MDM2 protein by western blotting in the appropriate cell lysate or extract.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: MDM2/HDM2
Long Name
Double Minute 2 Protein
Alternate Names
HDM2
Entrez Gene IDs
4193 (Human)
Gene Symbol
MDM2
UniProt
Additional MDM2/HDM2 Products
Product Documents for MDM2/HDM2 [p Ser185] Antibody
Product Specific Notices for MDM2/HDM2 [p Ser185] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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