MDR1/ABCB1 Antibody (A10E12-R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32685
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # A10E12-R
Concentration
1 mg/ml
Product Summary for MDR1/ABCB1 Antibody (A10E12-R)
Immunogen
Recombinant protein within human MDR1/ABCB1 aa 935-1,280 / 1,280. (Uniprot: P08183)
Localization
Cell membrane, Apical cell membrane, Cytoplasm.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
142 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for MDR1/ABCB1 Antibody (A10E12-R)
Immunohistochemistry: MDR1/ABCB1 Antibody (A10E12-R) [NBP3-32685]
Immunohistochemistry: MDR1/ABCB1 Antibody (A10E12-R) [NBP3-32685] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-MDR1/ABCB1 antibody (NBP3-32685) at 1/5,000 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32685) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Applications for MDR1/ABCB1 Antibody (A10E12-R)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MDR1/ABCB1
Long Name
ATP-binding Cassette, Sub-family B (MDR/TAP), Member 1B
Alternate Names
ABC20, ABCB1, ABCB1B, CD243, CLCS, GP170, IBD13, PGY1
Gene Symbol
ABCB1
Additional MDR1/ABCB1 Products
Product Documents for MDR1/ABCB1 Antibody (A10E12-R)
Product Specific Notices for MDR1/ABCB1 Antibody (A10E12-R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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