ME1 Antibody (15611)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43669

Novus Biologicals, part of Bio-Techne
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NBP2-43669
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (91%), Rat (91%), Sheep (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 15611

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ME1 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human ME1. The exact sequence is proprietary.

Reactivity Notes

Chicken (80%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

64 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ME1 Antibody (15611)

Western Blot: ME1 Antibody (15611) [NBP2-43669]

Western Blot: ME1 Antibody (15611) [NBP2-43669]

Western Blot: ME1 Antibody (15611) [NBP2-43669] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ME1 antibody [15611].
Immunohistochemistry-Frozen: ME1 Antibody (15611) [NBP2-43669]

Immunohistochemistry-Frozen: ME1 Antibody (15611) [NBP2-43669]

Immunohistochemistry-Frozen: ME1 Antibody (15611) [NBP2-43669] - Vimentin antibody (NBP1-31327) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488). Red: ME1 antibody 15611] (NBP2-43669) diluted at 1:200. The signal was developed using goat anti-mouse IgG antibody (Dylight594). Blue: Nuclear staining with Hoechst 33342.
Western Blot: ME1 Antibody (15611) [NBP2-43669]

Western Blot: ME1 Antibody (15611) [NBP2-43669]

Western Blot: ME1 Antibody (15611) [NBP2-43669] - Analysis of various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ME1 antibody diluted at a dilution of 1:1000.
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Applications for ME1 Antibody (15611)

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ME1

ME1 encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.

Alternate Names

EC 1.1.1, EC 1.1.1.40, HUMNDME, malate dehydrogenase, Malic enzyme 1, malic enzyme 1, NADP(+)-dependent, cytosolic, malic enzyme 1, soluble, Malic enzyme, cytoplasmic, MES, NADP-dependent malic enzyme, NADP-ME, pyruvic-malic carboxylase

Gene Symbol

ME1

Additional ME1 Products

Product Documents for ME1 Antibody (15611)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ME1 Antibody (15611)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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