ME1 Antibody (736)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43709
![Western Blot: ME1 Antibody (736) [NBP2-43709]](https://resources.bio-techne.com/images/products/ME1-Antibody-736-Western-Blot-NBP2-43709-img0002.jpg "Western Blot: ME1 Antibody (736) [NBP2-43709]")
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human
Predicted:
Bovine (91%), Mouse (91%), Rat (91%), Sheep (92%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 736
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human ME1. The exact sequence is proprietary.
Reactivity Notes
Chicken (80%).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
64 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ME1 Antibody (736)
![Immunocytochemistry/ Immunofluorescence: ME1 Antibody (736) [NBP2-43709]](https://resources.bio-techne.com/images/products/ME1-Antibody-736-Immunocytochemistry-Immunofluorescence-NBP2-43709-img0004.jpg "Immunocytochemistry/ Immunofluorescence: ME1 Antibody (736) [NBP2-43709]")
Immunocytochemistry/ Immunofluorescence: ME1 Antibody (736) [NBP2-43709]
Immunocytochemistry/Immunofluorescence: ME1 Antibody (736) [NBP2-43709] - HeLa cells were fixed in ice-cold MeOH for 5 min.Green: ME1 protein stained by ME1 antibody [736] diluted at 1:400. Blue: Hoechst 33342 staining.![Western Blot: ME1 Antibody (736) [NBP2-43709]](https://resources.bio-techne.com/images/products/ME1-Antibody-736-Western-Blot-NBP2-43709-img0001.jpg "Western Blot: ME1 Antibody (736) [NBP2-43709]")
Western Blot: ME1 Antibody (736) [NBP2-43709]
Western Blot: ME1 Antibody (736) [NBP2-43709] - Analysis of various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ME1 antibody [736] diluted at a dilution of 1:500.Applications for ME1 Antibody (736)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: ME1
Alternate Names
EC 1.1.1, EC 1.1.1.40, HUMNDME, malate dehydrogenase, Malic enzyme 1, malic enzyme 1, NADP(+)-dependent, cytosolic, malic enzyme 1, soluble, Malic enzyme, cytoplasmic, MES, NADP-dependent malic enzyme, NADP-ME, pyruvic-malic carboxylase
Gene Symbol
ME1
Additional ME1 Products
Product Documents for ME1 Antibody (736)
Product Specific Notices for ME1 Antibody (736)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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