ME2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82434

Novus Biologicals, part of Bio-Techne
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NBP1-82434
NBP1-82434-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ME2 Antibodies »

Product Summary for ME2 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DGRVFTPGQGNNVYIFPGVALAVILCNTRHISDSVFLEAAKALTSQLTDEELAQGRLYPPLANIQEVSINIAIKVTEYLYANKMAFRYPEPEDKAKYVKERTWRSEYDSLLPDVYEWPESASSPPV

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%), Rat (87%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ME2 Antibody

Western Blot: ME2 Antibody [NBP1-82434]

Western Blot: ME2 Antibody [NBP1-82434]

Western Blot: ME2 Antibody [NBP1-82434] - Analysis using Anti-ME2 antibody NBP1-82434 (A) shows similar pattern to independent antibody NBP1-89559 (B).
Immunocytochemistry/ Immunofluorescence: ME2 Antibody [NBP1-82434]

Immunocytochemistry/ Immunofluorescence: ME2 Antibody [NBP1-82434]

Immunocytochemistry/Immunofluorescence: ME2 Antibody [NBP1-82434] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
Immunohistochemistry-Paraffin: ME2 Antibody [NBP1-82434]

Immunohistochemistry-Paraffin: ME2 Antibody [NBP1-82434]

Immunohistochemistry-Paraffin: ME2 Antibody [NBP1-82434] - Staining of human cerebral cortex.
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Applications for ME2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ME2

The ME2 gene encodes a mitochondrial NAD-dependent malic enzyme, a homotetrameric protein, that catalyzes the oxidative decarboxylation of malate to pyruvate. It had previously been weakly linked to a syndrome known as Friedreich ataxia that has since been s

Alternate Names

EC 1.1.1, EC 1.1.1.38, malate dehydrogenase, Malic enzyme 2, malic enzyme 2, NAD(+)-dependent, mitochondrial, NAD-dependent malic enzyme, mitochondrial, NAD-ME, ODS1, pyruvic-malic carboxylase

Gene Symbol

ME2

Additional ME2 Products

Product Documents for ME2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ME2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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