MECR Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85657

Novus Biologicals, part of Bio-Techne
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NBP1-85657
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MECR Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SSTELLRQLARGGTMVTYGGMAKQPVVASVSLLIFKDLKLRGFWLSQWKKDHSPDQFKELILTLCDLIRRGQLTAPACSQVPLQDYQSALEASMKPFISSKQILTM

Reactivity Notes

Rat (87%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MECR Antibody

Western Blot: MECR Antibody [NBP1-85657]

Western Blot: MECR Antibody [NBP1-85657]

Western Blot: MECR Antibody [NBP1-85657] - Analysis using Anti-MECR antibody NBP1-85657 (A) shows similar pattern to independent antibody NBP1-85659 (B).
Immunocytochemistry/ Immunofluorescence: MECR Antibody [NBP1-85657]

Immunocytochemistry/ Immunofluorescence: MECR Antibody [NBP1-85657]

Immunocytochemistry/Immunofluorescence: MECR Antibody [NBP1-85657] - Immunofluorescent staining of human cell line A-431 shows localization to mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MECR Antibody [NBP1-85657]

Immunohistochemistry-Paraffin: MECR Antibody [NBP1-85657]

Immunohistochemistry-Paraffin: MECR Antibody [NBP1-85657] - Staining of human testis.
View 6 more images

Applications for MECR Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MECR

MECR catalyzes the reversible phosphorylytic cleavage of uridine and deoxyuridine to uracil and ribose- ordeoxyribose-1-phosphate. The produced molecules are then utilized as carbon and energy sources or in the rescue ofpyrimidine bases for nucleotide synthesi

Alternate Names

CGI-63, EC 1.3.1.38, FASN2B, homolog of yeast 2-enoyl thioester reductase, HsNrbf-1, mitochondrial 2-enoyl thioester reductase, mitochondrial trans-2-enoyl-CoA reductase, NBRF1, NRBF-1, NRBF1hsNrbf-1, nuclear receptor binding factor 1, Nuclear receptor-binding factor 1, trans-2-enoyl-CoA reductase, mitochondrial

Gene Symbol

MECR

Additional MECR Products

Product Documents for MECR Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MECR Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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