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MED10 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81328

Novus Biologicals, part of Bio-Techne
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NBP1-81328
NBP1-81328-25ul

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for MED10 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: FEYIDQGRNPQLYTKECLERALAKNEQVKGKIDTMKKFKSLLIQELSKVFPEDMAKYRSIRGEDHPPS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MED10 Antibody

Western Blot: MED10 Antibody [NBP1-81328]

Western Blot: MED10 Antibody [NBP1-81328]

Western Blot: MED10 Antibody [NBP1-81328] - Analysis in control (vector only transfected HEK293T lysate) and MED10 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: MED10 Antibody [NBP1-81328]

Immunocytochemistry/ Immunofluorescence: MED10 Antibody [NBP1-81328]

Immunocytochemistry/Immunofluorescence: MED10 Antibody [NBP1-81328] - Staining of human cell line PC-3 shows localization to nucleoplasm. Antibody staining is shown in green.

Applications for MED10 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
Recommended conditions: Fixation/Permeabilization: PFA/Triton X-100
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MED10

MED10 is a component of the Mediator complex, which is a coactivator for DNA-binding factors that activatetranscription via RNA polymerase II (Sato et al., 2003 (PubMed 12584197)).(supplied by OMIM)

Alternate Names

L6, mediator complex subunit 10TRG-17, mediator of RNA polymerase II transcription subunit 10, mediator of RNA polymerase II transcription, subunit 10, mediator of RNA polymerase II transcription, subunit 10 homolog (NUT2, S.cerevisiae), MGC5309, NUT2TRG-20, Transformation-related gene 17 protein, Transformation-related gene 20 protein, TRG20

Gene Symbol

MED10

Additional MED10 Products

Product Documents for MED10 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MED10 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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