MED13 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-60641

Novus Biologicals, part of Bio-Techne
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NB100-60641
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all MED13 Antibodies »

Product Summary for MED13 Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residue 325 and 375 of human thyroid hormone receptor associated protein 1 (thyroid hormone receptor-associated protein, 240 kDa) using the numbering given in entry NP_005112.2 (GeneID 996

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MED13 Antibody

Western Blot: MED13 Antibody [NB100-60641]

Western Blot: MED13 Antibody [NB100-60641]

Western Blot: MED13 Antibody [NB100-60641] - Detection of Human THRAP1/TRAP240 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-THRAP1/TRAP240 antibody NB100-60641 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
MED13 Antibody [NB100-60641] - Detection of human THRAP1/TRAP240 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-THRAP1/TRAP240 antibody A301-277A (lot NB100-60641-2) used for IP at 6 ug per reaction. THRAP1/TRAP240 was also immunoprecipitated by a previous lot of this antibody (lot NB100-60641-1) and another rabbit anti-THRAP1/TRAP240 antibody. For blotting immunoprecipitated THRAP1/TRAP240, NB100-60641 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for MED13 Antibody

Application
Recommended Usage

Immunoprecipitation

1:10-1:500

Western Blot

1:2000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MED13

This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. The product of this gene is proposed to form a sub-complex with MED12, cyclin C, and CDK8 that can negatively regulate transactivation by mediator.

Alternate Names

Activator-recruited cofactor 250 kDa component, KIAA0593HSPC221, mediator complex subunit 13ARC250, mediator of RNA polymerase II transcription, subunit 13 homolog, THRAP1mediator of RNA polymerase II transcription subunit 13, thyroid hormone receptor associated protein 1, Thyroid hormone receptor-associated protein 1, Thyroid hormone receptor-associated protein complex 240 kDa component, thyroid hormone receptor-associated protein complex component TRAP240, thyroid hormone receptor-associated protein, 240 kDa subunit, Trap240, TRAP240DRIP250, Vitamin D3 receptor-interacting protein complex component DRIP250

Entrez Gene IDs

9969 (Human)

Gene Symbol

MED13

UniProt

NP_005112.2 (Human)

Additional MED13 Products

Product Documents for MED13 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MED13 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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