Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all MED16 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 550 and 600 of human Mediator Complex Subunit 16 using the numbering given in entry NP_005472.2 (GeneID 10025).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MED16 Antibody

Immunoprecipitation: MED16 Antibody [NBP2-04141]

Immunoprecipitation: MED16 Antibody [NBP2-04141]

Immunoprecipitation: MED16 Antibody [NBP2-04141] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-MED16 antibody NBP2-04141 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. MED16 was also immunoprecipitated by rabbit anti-MED16 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Applications for MED16 Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MED16

MED16 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymera

Alternate Names

DRIP92thyroid hormone receptor associated protein 5, mediator complex subunit 16THRAP5, mediator of RNA polymerase II transcription subunit 16, Thyroid hormone receptor-associated protein 5, Thyroid hormone receptor-associated protein complex 95 kDa component, thyroid hormone receptor-associated protein, 95-kD subunit, TRAP95, Vitamin D3 receptor-interacting protein complex 92 kDa component

Gene Symbol

MED16

UniProt

NP_005472.2 (Human)

Additional MED16 Products

Product Documents for MED16 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MED16 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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