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MED18 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83673

Novus Biologicals, part of Bio-Techne
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NBP1-83673
NBP1-83673-25ul

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RARRSMDRAGAPWHLRYLGQPEMGDKNRHALVRNCVDIATSENLTDFLMEMGFRMDHEFVAKGH

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MED18 Antibody

Immunocytochemistry/ Immunofluorescence: MED18 Antibody [NBP1-83673]

Immunocytochemistry/ Immunofluorescence: MED18 Antibody [NBP1-83673]

Immunocytochemistry/Immunofluorescence: MED18 Antibody [NBP1-83673] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: MED18 Antibody [NBP1-83673]

Immunohistochemistry-Paraffin: MED18 Antibody [NBP1-83673]

Immunohistochemistry-Paraffin: MED18 Antibody [NBP1-83673] - Staining of human colon shows moderate nuclear and cytoplasmic positivity in glandular cells.

Applications for MED18 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MED18

MED18 is a component of the Mediator complex, which is a coactivator for DNA-binding factors that activate transcription via RNA polymerase II (Sato et al., 2003 [PubMed 12584197]).[supplied by OMIM]

Alternate Names

FLJ20045, mediator complex subunit 18mediator of RNA polymerase II transcription subunit 18, mediator of RNA polymerase II transcription, subunit 18 homolog (S. cerevisiae), p28bmediator of RNA polymerase II transcription, subunit 18 homolog, TRAP/mediator complex subunit p28b

Gene Symbol

MED18

Additional MED18 Products

Product Documents for MED18 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MED18 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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