MED18 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-17257

Novus Biologicals, part of Bio-Techne
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NBP2-17257
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MED18 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human MED18. The exact sequence is proprietary.

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MED18 Antibody

Western Blot: MED18 Antibody [NBP2-17257]

Western Blot: MED18 Antibody [NBP2-17257]

Western Blot: MED18 Antibody [NBP2-17257] - Sample (30 ug of whole cell lysate) A: A549 12% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: MED18 Antibody [NBP2-17257]

Immunocytochemistry/ Immunofluorescence: MED18 Antibody [NBP2-17257]

Immunocytochemistry/Immunofluorescence: MED18 Antibody [NBP2-17257] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: MED18 Antibody [NBP2-17257]

Immunohistochemistry-Paraffin: MED18 Antibody [NBP2-17257]

Immunohistochemistry-Paraffin: MED18 Antibody [NBP2-17257] - Cal27 xenograft, using MED18 antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Applications for MED18 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MED18

MED18 is a component of the Mediator complex, which is a coactivator for DNA-binding factors that activate transcription via RNA polymerase II (Sato et al., 2003 [PubMed 12584197]).[supplied by OMIM]

Alternate Names

FLJ20045, mediator complex subunit 18mediator of RNA polymerase II transcription subunit 18, mediator of RNA polymerase II transcription, subunit 18 homolog (S. cerevisiae), p28bmediator of RNA polymerase II transcription, subunit 18 homolog, TRAP/mediator complex subunit p28b

Gene Symbol

MED18

UniProt

Q9BUE0 (Human)

Additional MED18 Products

Product Documents for MED18 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MED18 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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