MED20 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # H00009477-B01P

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Mouse IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MED20 Antibodies »

Product Specifications

Immunogen

MED20 (NP_004266.2, 1 a.a. - 212 a.a.) full-length human protein. MGVTCVSQMPVAEGKSVQQTVELLTRKLEMLGAEKQGTFCVDCETYHTAASTLGSQGQTGKLMYVMHNSEYPLSCFALFENGPCLIADTNFDVLMVKLKGFFQSAKASKIETRGTRYQYCDFLVKVGTVTMGPSARGISVEVEYGPCVVASDCWSLLLEFLQSFLGSHTPGAPAVFGNRHDAVYGPADTMVQYMELFNKIRKQQQVPVAGIR

Specificity

MED20 - mediator complex subunit 20,

Clonality

Polyclonal

Host

Mouse

Isotype

IgG

Scientific Data Images for MED20 Antibody

Western Blot: MED20 Antibody [H00009477-B01P]

Western Blot: MED20 Antibody [H00009477-B01P]

Western Blot: MED20 Antibody [H00009477-B01P] - Analysis of TRFP expression in Hela S3 NE.
Immunocytochemistry/ Immunofluorescence: MED20 Antibody [H00009477-B01P]

Immunocytochemistry/ Immunofluorescence: MED20 Antibody [H00009477-B01P]

Immunocytochemistry/Immunofluorescence: MED20 Antibody [H00009477-B01P] - Analysis of purified antibody to TRFP on HeLa cell. (antibody concentration 10 ug/ml)
Western Blot: MED20 Antibody [H00009477-B01P]

Western Blot: MED20 Antibody [H00009477-B01P]

Western Blot: MED20 Antibody [H00009477-B01P] - Analysis of MED20 expression in transfected 293T cell line by MED20 polyclonal antibody. Lane 1: TRFP transfected lysate(23.32 KDa). Lane 2: Non-transfected lysate.

Applications for MED20 Antibody

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control. It is also useful for immunofluoresence.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MED20

MED20 is a subunit of the Mediator complex, a multiprotein coactivator of RNA transcription that interacts with DNA-bound transcriptional activators, RNA polymerase II (see MIM 180660), and general initiation factors (Sato et al., 2003 (PubMed 14576168)).(supplied by OMIM)

Alternate Names

hTRFP, mediator complex subunit 20MGC29869, mediator of RNA polymerase II transcription subunit 20, PRO0213, Trf (TATA binding protein-related factor)-proximal homolog, Trf (TATA binding protein-related factor)-proximal homolog (Drosophila), TRFPDKFZp586D2223, TRF-proximal protein homolog

Entrez Gene IDs

9477 (Human)

Gene Symbol

MED20

UniProt

NP_004266.2 (Human)

Additional MED20 Products

Product Documents for MED20 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MED20 Antibody

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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