MED20 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84140

Novus Biologicals, part of Bio-Techne
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NBP1-84140
NBP1-84140-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%), Rat (99%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MED20 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EKQGTFCVDCETYHTAASTLGSQGQTGKLMYVMHNSEYPLSCFALFENGPCLIADTNFDVLMVKLKGFFQSAKASKIETRGTRYQYCDFLVKVGTVTMGPSARGISVEVEYGPCVV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MED20 Antibody

Immunocytochemistry/ Immunofluorescence: MED20 Antibody [NBP1-84140]

Immunocytochemistry/ Immunofluorescence: MED20 Antibody [NBP1-84140]

Immunocytochemistry/Immunofluorescence: MED20 Antibody [NBP1-84140] - Staining of human cell line U-251 MG shows localization to nucleoplasm & centrosome. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MED20 Antibody [NBP1-84140]

Immunohistochemistry-Paraffin: MED20 Antibody [NBP1-84140]

Immunohistochemistry-Paraffin: MED20 Antibody [NBP1-84140] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: MED20 Antibody [NBP1-84140]

Immunohistochemistry-Paraffin: MED20 Antibody [NBP1-84140]

Immunohistochemistry-Paraffin: MED20 Antibody [NBP1-84140] - Staining of human endometrium shows moderate nuclear positivity.
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Applications for MED20 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval method is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MED20

MED20 is a subunit of the Mediator complex, a multiprotein coactivator of RNA transcription that interacts with DNA-bound transcriptional activators, RNA polymerase II (see MIM 180660), and general initiation factors (Sato et al., 2003 (PubMed 14576168)).(supplied by OMIM)

Alternate Names

hTRFP, mediator complex subunit 20MGC29869, mediator of RNA polymerase II transcription subunit 20, PRO0213, Trf (TATA binding protein-related factor)-proximal homolog, Trf (TATA binding protein-related factor)-proximal homolog (Drosophila), TRFPDKFZp586D2223, TRF-proximal protein homolog

Gene Symbol

MED20

Additional MED20 Products

Product Documents for MED20 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MED20 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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