MED21 Antibody (5A6)
Novus Biologicals, part of Bio-Techne | Catalog # H00009412-M04
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 5A6
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
SURB7 (AAH08380, 1 a.a. ~ 144 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MADRLTQLQDAVNSLADQFCNAIGVLQQCGPPASFNNIQTAINKDQPANPTEEYAQLFAALIARTAKDIDVLIDSLPSEESTAALQAASLYKLEEENHEAATCLEDVVYRGDMLLEKIQSALADIAQSQLKTRSGTHSQSLPDS
Reactivity Notes
Human. Other species not tested.
Specificity
SURB7 - SRB7 suppressor of RNA polymerase B homolog (yeast)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for MED21 Antibody (5A6)
![Western Blot: MED21 Antibody (5A6) [H00009412-M04]](https://resources.bio-techne.com/images/products/MED21-Antibody-5A6-Western-Blot-H00009412-M04-img0007.jpg "Western Blot: MED21 Antibody (5A6) [H00009412-M04]")
Western Blot: MED21 Antibody (5A6) [H00009412-M04]
Western Blot: MED21 Antibody (5A6) [H00009412-M04] - Analysis of SURB7 expression in HeLa (Cat # L013V1).![Immunocytochemistry/ Immunofluorescence: MED21 Antibody (5A6) [H00009412-M04]](https://resources.bio-techne.com/images/products/MED21-Antibody-5A6-Immunocytochemistry-Immunofluorescence-H00009412-M04-img0005.jpg "Immunocytochemistry/ Immunofluorescence: MED21 Antibody (5A6) [H00009412-M04]")
Immunocytochemistry/ Immunofluorescence: MED21 Antibody (5A6) [H00009412-M04]
Immunocytochemistry/Immunofluorescence: MED21 Antibody (5A6) [H00009412-M04] - Analysis of monoclonal antibody to SURB7 on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: MED21 Antibody (5A6) [H00009412-M04] - Detection limit for recombinant GST tagged SURB7 is approximately 0.03ng/ml as a capture antibody.
Applications for MED21 Antibody (5A6)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: MED21
Alternate Names
hSrb7, mediator complex subunit 21SRB7 (suppressor of RNA polymerase B, yeast) homolog, RNA polymerase II complex component SRB7, RNA polymerase II holoenzyme component SRB7, RNAPII complex component SRB7, SRB7 suppressor of RNA polymerase B homolog, SRB7SRB7 suppressor of RNA polymerase B homolog (yeast), SURB7mediator of RNA polymerase II transcription subunit 21
Entrez Gene IDs
9412 (Human)
Gene Symbol
MED21
OMIM
603800 (Human)
UniProt
Additional MED21 Products
Product Documents for MED21 Antibody (5A6)
Product Specific Notices for MED21 Antibody (5A6)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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