MED21 Antibody (PCRP-MED21-4B5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13814

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # PCRP-MED21-4B5

Concentration

0.2 mg/ml

View all MED21 Antibodies »

Product Specifications

Immunogen

Recombinant full-length human MED21 protein (Uniprot: Q13503)

Localization

Nucleus, Nucleoplasm, Cytoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

15.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14069)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for MED21 Antibody (PCRP-MED21-4B5)

Immunocytochemistry/ Immunofluorescence: MED21 Antibody (PCRP-MED21-4B5) [NBP3-13814]

Immunocytochemistry/ Immunofluorescence: MED21 Antibody (PCRP-MED21-4B5) [NBP3-13814]

Immunocytochemistry/Immunofluorescence: MED21 Antibody (PCRP-MED21-4B5) [NBP3-13814] - Immunofluorescence Analysis of PFA-fixed U87 cells stained using MED21 Antibody (PCRP-MED21-4B5) followed by goat anti-mouse IgG-CF488. Membrane stained with phalloidin.
Flow Cytometry: MED21 Antibody (PCRP-MED21-4B5) [NBP3-13814]

Flow Cytometry: MED21 Antibody (PCRP-MED21-4B5) [NBP3-13814]

Flow Cytometry: MED21 Antibody (PCRP-MED21-4B5) [NBP3-13814] - Flow cytometric analysis of PFA-fixed U87 cells. MED21 antibody (PCRP-MED21-4B5) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).
Immunocytochemistry/ Immunofluorescence: MED21 Antibody (PCRP-MED21-4B5) [NBP3-13814]

Immunocytochemistry/ Immunofluorescence: MED21 Antibody (PCRP-MED21-4B5) [NBP3-13814]

Immunocytochemistry/Immunofluorescence: MED21 Antibody (PCRP-MED21-4B5) [NBP3-13814] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using MED21 Antibody (PCRP-MED21-4B5) followed by goat anti-mouse IgG-CF488. Membrane stained with phalloidin.
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Applications for MED21 Antibody (PCRP-MED21-4B5)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunoprecipitation

1-2 ug/100-500 ug protein

Western Blot

1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: MED21

MED21 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymera

Alternate Names

hSrb7, mediator complex subunit 21SRB7 (suppressor of RNA polymerase B, yeast) homolog, RNA polymerase II complex component SRB7, RNA polymerase II holoenzyme component SRB7, RNAPII complex component SRB7, SRB7 suppressor of RNA polymerase B homolog, SRB7SRB7 suppressor of RNA polymerase B homolog (yeast), SURB7mediator of RNA polymerase II transcription subunit 21

Gene Symbol

MED21

UniProt

Q13503 (Human)

Additional MED21 Products

Product Documents for MED21 Antibody (PCRP-MED21-4B5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MED21 Antibody (PCRP-MED21-4B5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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