MED25 Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-04944
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for MED25 Antibody - Azide and BSA Free
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 30-200 of human MED25 (NP_112235.2). PYFEGLRKHYLLPAIEYFNGGPPAETDFGGDYGGTQYSLVVFNTVDCAPESYVQCHAPTSSAYEFVTWLDGIKFMGGGGESCSLIAEGLSTALQLFDDFKKMREQIGQTHRVCLLICNSPPYLLPAVESTTYSGCTTENLVQQIGERGIHFSIVSPRKLPALRLLFEKAAP
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for MED25 Antibody - Azide and BSA Free
![Western Blot: MED25 AntibodyAzide and BSA Free [NBP3-04944]](https://resources.bio-techne.com/images/products/MED25-Antibody-Western-Blot-NBP3-04944-img0002.jpg "Western Blot: MED25 AntibodyAzide and BSA Free [NBP3-04944]")
Western Blot: MED25 AntibodyAzide and BSA Free [NBP3-04944]
Western Blot: MED25 Antibody [NBP3-04944] - Analysis of extracts of various cell lines, using MED25 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit![Immunocytochemistry/ Immunofluorescence: MED25 Antibody - Azide and BSA Free [NBP3-04944]](https://resources.bio-techne.com/images/products/MED25-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-04944-img0001.jpg "Immunocytochemistry/ Immunofluorescence: MED25 Antibody - Azide and BSA Free [NBP3-04944]")
Immunocytochemistry/ Immunofluorescence: MED25 Antibody - Azide and BSA Free [NBP3-04944]
Immunocytochemistry/Immunofluorescence: MED25 Antibody [NBP3-04944] - Analysis of U-2 OS cells using MED25 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Applications for MED25 Antibody - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS with 50% glycerol, pH7.3.
Format
Azide and BSA Free
Preservative
0.01% Thimerosal
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: MED25
Alternate Names
ACID1, Activator Interaction Domain-Containing Protein 1, Activator-Recruited Cofactor 92 KDa Component, ARC/Mediator Transcriptional Coactivator Subunit, ARC92, BVSYS, CMT2B2, Mediator Complex Subunit 25, Mediator Of RNA Polymerase II Transcription Subunit 25, Mediator Of RNA Polymerase II Transcription, Subunit 25 Homolog, Mediator Of RNA Polymerase II Transcription, Subunit 25 Homolog (S. Cerevisiae), P78, TCBAP0758
Gene Symbol
MED25
Additional MED25 Products
Product Documents for MED25 Antibody - Azide and BSA Free
Product Specific Notices for MED25 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov