MED31 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-56861
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Chromatin Immunoprecipitation (ChIP), Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Synthetic peptides corresponding to MED31(mediator complex subunit 31) The peptide sequence was selected from the N terminal of MED31.
Peptide sequence MAAAVAMETDDAGNRLRFQLELEFVQCLANPNYLNFLAQRGYFKDKAFVN. The peptide sequence for this immunogen was taken from within the described region.
Specificity
This product is specific to Subunit or Isoform: 31.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Scientific Data Images for MED31 Antibody
![Western Blot: MED31 Antibody [NBP1-56861]](https://resources.bio-techne.com/images/products/MED31-Antibody-Western-Blot-NBP1-56861-img0003.jpg "Western Blot: MED31 Antibody [NBP1-56861]")
Western Blot: MED31 Antibody [NBP1-56861]
Western Blot: MED31 Antibody [NBP1-56861] - Quiescent human colon carcinoma cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.![Western Blot: MED31 Antibody [NBP1-56861]](https://resources.bio-techne.com/images/products/MED31-Antibody-Western-Blot-NBP1-56861-img0002.jpg "Western Blot: MED31 Antibody [NBP1-56861]")
Western Blot: MED31 Antibody [NBP1-56861]
Western Blot: MED31 Antibody [NBP1-56861] - Human Liver cell lysate, concentration 0.2-1 ug/ml.Applications for MED31 Antibody
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
1:10-1:500
Western Blot
1.0 ug/ml
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MED31
Alternate Names
CGI-125, FLJ27436, FLJ36714, hSOH1, mediator complex subunit 313110004H13Rik, Mediator complex subunit SOH1, mediator of RNA polymerase II transcription subunit 31, mediator of RNA polymerase II transcription, subunit 31 homolog, mediator of RNA polymerase II transcription, subunit 31 homolog (S. cerevisiae), Soh1
Gene Symbol
MED31
UniProt
Additional MED31 Products
Product Documents for MED31 Antibody
Product Specific Notices for MED31 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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