MEF2A Antibody (3F6)

Novus Biologicals, part of Bio-Techne | Catalog # H00004205-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 3F6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MEF2A Antibodies »

Product Specifications

Immunogen

MEF2A (AAH13437, 71 a.a. ~ 170 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EYNEPHESRTNSDIVEALNKKEHRGCDSPDPDTSYVLTPHTEEKYKKINEEFDNMMRNHKIAPGLPPQNFSMSVTVPVTSPNALSYTNPGSSLVSPSLAA

Specificity

MADS box transcription enhancer factor 2, polypeptide A (myocyte enhancer factor 2A)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for MEF2A Antibody (3F6)

Immunohistochemistry-Paraffin: MEF2A Antibody (3F6) [H00004205-M01]

Immunohistochemistry-Paraffin: MEF2A Antibody (3F6) [H00004205-M01]

Immunohistochemistry-Paraffin: MEF2A Antibody (3F6) [H00004205-M01] - Analysis of monoclonal antibody to MEF2A on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.
Sandwich ELISA: MEF2A Antibody (3F6) [H00004205-M01] - Detection limit for recombinant GST tagged MEF2A is approximately 1ng/ml as a capture antibody.

Applications for MEF2A Antibody (3F6)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for IHC-P and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MEF2A

The process of differentiation from mesodermal precursor cells to myoblasts has led to the discovery of a variety of tissue-specific factors that regulate muscle gene expression. The myogenic basic helix-loop-helix proteins, including myoD (MIM 159970), myogenin (MIM 159980), MYF5 (MIM 159990), and MRF4 (MIM 159991) are one class of identified factors. A second family of DNA binding regulatory proteins is the myocyte-specific enhancer factor-2 (MEF2) family. Each of these proteins binds to the MEF2 target DNA sequence present in the regulatory regions of many, if not all, muscle-specific genes. The MEF2 genes are members of the MADS gene family (named for the yeast mating type-specific transcription factor MCM1, the plant homeotic genes 'agamous' and 'deficiens' and the human serum response factor SRF (MIM 600589)), a family that also includes several homeotic genes and other transcription factors, all of which share a conserved DNA-binding domain.[supplied by OMIM]

Alternate Names

ADCAD1, MADS box transcription enhancer factor 2, polypeptide A (myocyte enhancerfactor 2A), MEF2, myocyte enhancer factor 2A, myocyte-specific enhancer factor 2A, RSRFC4, RSRFC9, Serum response factor-like protein 1

Entrez Gene IDs

4205 (Human)

Gene Symbol

MEF2A

OMIM

600660 (Human)

UniProt

AAH13437 (Human)

Additional MEF2A Products

Product Documents for MEF2A Antibody (3F6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MEF2A Antibody (3F6)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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