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MEF2C Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89468

Novus Biologicals, part of Bio-Techne
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NBP1-89468
NBP1-89468-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (99%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PPNFEMPVSIPVSSHNSLVYSNPVSSLGNPNLLPLAHPSLQRNSMSPGVTHRPPSAGNTGGLMGGDLTSGAGTSAGNGYGNPRNSPGLLVSPGNLNKNMQAKSPPPMNLGMNNRKPDLRVLIPPGSKNTMPSVNQRINN

Reactivity Notes

Reactivity reported in scientific literature (PMID: 21163264).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MEF2C Antibody

Immunohistochemistry-Paraffin: MEF2C Antibody [NBP1-89468]

Immunohistochemistry-Paraffin: MEF2C Antibody [NBP1-89468]

Immunohistochemistry-Paraffin: MEF2C Antibody [NBP1-89468] - Analysis of human cerebral cortex and liver tissues. Corresponding MEF2C RNA-seg data are presented for the same tissues.
Western Blot: MEF2C Antibody [NBP1-89468]

Western Blot: MEF2C Antibody [NBP1-89468]

Western Blot: MEF2C Antibody [NBP1-89468] - Analysis in control (vector only transfected HEK293T lysate) and MEF2C over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: MEF2C Antibody [NBP1-89468]

Immunocytochemistry/ Immunofluorescence: MEF2C Antibody [NBP1-89468]

Immunocytochemistry/Immunofluorescence: MEF2C Antibody [NBP1-89468] - Staining of human cell line RH-30 shows localization to nucleoplasm & vesicles. Antibody staining is shown in green.

Applications for MEF2C Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MEF2C

Transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. Controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. May also be involved in neurogenesis and in the development of cortical architecture . Isoform 3 and isoform 4, which lack the repressor domain, are more active than isoform 1 and isoform 2

Long Name

Myocyte-specific Enhancer Factor 2C

Alternate Names

DEL5q14.3

Gene Symbol

MEF2C

Additional MEF2C Products

Product Documents for MEF2C Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MEF2C Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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