MEF2D Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85788

Novus Biologicals, part of Bio-Techne
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NBP1-85788
NBP1-85788-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MEF2D Antibodies »

Product Summary for MEF2D Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QPQPPQQQPPQPQQPQPQQPQQPQQPPQQQSHLVPVSLSNLIPGSPLPHVGAALTVTTHPHISIKSEPVSPSRERSPAPPPPAVFPAARPEPGDGLSSPAGGSYETGDRDDGRGDFGPTLGLLRPAPEPEAEGSAVKRMRLDTWTL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MEF2D Antibody

Immunohistochemistry-Paraffin: MEF2D Antibody [NBP1-85788]

Immunohistochemistry-Paraffin: MEF2D Antibody [NBP1-85788]

Immunohistochemistry-Paraffin: MEF2D Antibody [NBP1-85788] - Analysis in human skeletal muscle and liver tissues using NBP1-85788 antibody. Corresponding MEF2D RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: MEF2D Antibody [NBP1-85788]

Immunohistochemistry-Paraffin: MEF2D Antibody [NBP1-85788]

Immunohistochemistry-Paraffin: MEF2D Antibody [NBP1-85788] - Staining of human kidney, liver, placenta and skeletal muscle using Anti-MEF2D antibody NBP1-85788 (A) shows similar protein distribution across tissues to independent antibody NBP1-85795 (B).
Western Blot: MEF2D Antibody [NBP1-85788]

Western Blot: MEF2D Antibody [NBP1-85788]

Western Blot: MEF2D Antibody [NBP1-85788] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
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Applications for MEF2D Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Simple Western

reported by internal validation

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. MEF2D antibody validated for ELISA from a verified customer review. In Simple Western internal validation: Mouse Brain lysate as sample; separated by size; antibody dilution of 1:50 - 1:1000; matrix was 12-230 kDa; detected by Chemiluminescence.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4 using NBP1-85788 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MEF2D

Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific, growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. Plays a critical role in the regulation of neuronal apoptosis

Alternate Names

DKFZp686I1536, MADS box transcription enhancer factor 2, polypeptide D (myocyte enhancerfactor 2D), MEF2D/DAZAP1 fusion, myocyte enhancer factor 2D, myocyte enhancer factor 2D/deleted in azoospermia associated protein 1 fusionprotein, myocyte-specific enhancer factor 2D

Gene Symbol

MEF2D

Additional MEF2D Products

Product Documents for MEF2D Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MEF2D Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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