MEK1 Antibody (3F10)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26152
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 3F10
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from Human MEK1 [UniProt Q02750]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for MEK1 Antibody (3F10)
Immunoprecipitation: MEK1 Antibody (3F10) [NBP3-26152] -
Immunoprecipitation: MEK1 Antibody (3F10) [NBP3-26152] - Immunoprecipitating MEK1 in Hela whole cell lysate.Lane 1: Rabbit control IgG instead of NBP3-26152 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).
Lane 2: NBP3-26152 (2ug) + Hela whole cell lysate (500ug).
Lane 3: Hela whole cell lysate (10ug).
Immunohistochemistry: MEK1 Antibody (3F10) [NBP3-26152] -
Immunohistochemistry: MEK1 Antibody (3F10) [NBP3-26152] - Image of MEK1 Antibody (3F10) diluted at 1:100 and staining in paraffin-embedded human colon cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Western Blot: MEK1 Antibody (3F10) [NBP3-26152] -
Western Blot: MEK1 Antibody (3F10) [NBP3-26152] - Positive Western Blot detected in: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, U87 whole cell lysate.All lanes: MEK1 Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 44, 41 kDa
Observed band size: 44 kDa
Applications for MEK1 Antibody (3F10)
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Immunoprecipitation
1:200-1:1000
Western Blot
1:500-1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: MEK1
Long Name
Mitogen-activated Protein Kinase Kinase 1
Alternate Names
MAP2K1, MKK1
Gene Symbol
MAP2K1
Additional MEK1 Products
Product Documents for MEK1 Antibody (3F10)
Product Specific Notices for MEK1 Antibody (3F10)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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