MEK1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87790
![Immunohistochemistry-Paraffin: MEK1 Antibody [NBP1-87790]](https://resources.bio-techne.com/images/products/MEK1-Antibody-Immunohistochemistry-Paraffin-NBP1-87790-img0001.jpg "Immunohistochemistry-Paraffin: MEK1 Antibody [NBP1-87790]")
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Predicted:
Mouse (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: MPKKKPTPIQLNPAPDGSAVNGTSSAETNLEALQKKLEELELDEQQRKRLEAFLTQKQ
Reactivity Notes
Human reactivity reported in scientific literature (PMID: 25408231).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for MEK1 Antibody
![Western Blot: MEK1 Antibody [NBP1-87790]](https://resources.bio-techne.com/images/products/MEK1-Antibody-Western-Blot-NBP1-87790-img0009.jpg "Western Blot: MEK1 Antibody [NBP1-87790]")
Western Blot: MEK1 Antibody [NBP1-87790]
Western Blot: MEK1 Antibody [NBP1-87790] - Analysis in human cell line U-87 MG.![Immunocytochemistry/ Immunofluorescence: MEK1 Antibody [NBP1-87790]](https://resources.bio-techne.com/images/products/MEK1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87790-img0007.jpg "Immunocytochemistry/ Immunofluorescence: MEK1 Antibody [NBP1-87790]")
Immunocytochemistry/ Immunofluorescence: MEK1 Antibody [NBP1-87790]
Immunocytochemistry/Immunofluorescence: MEK1 Antibody [NBP1-87790] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.![Immunohistochemistry: MEK1 Antibody [NBP1-87790]](https://resources.bio-techne.com/images/products/MEK1-Antibody-Immunohistochemistry-NBP1-87790-img0008.jpg "Immunohistochemistry: MEK1 Antibody [NBP1-87790]")
Immunohistochemistry: MEK1 Antibody [NBP1-87790]
Immunohistochemistry: MEK1 Antibody [NBP1-87790] - Correlation between MEK1 expression and survival after platinum treatment in EOC patients. Representative images of immunohistochemistry, low and high expression of MEK1 at low and high magnifications. Image collected and cropped by CiteAb from the following publication (https://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-14-837), licensed under a CC-BY license.Applications for MEK1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:20 - 1:50
Immunohistochemistry-Paraffin
1:20 - 1:50
Western Blot
0.04 - 0.4 ug/ml
Application Notes
IHC reported in scientific literature (PMID: 25408231). For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MEK1
Long Name
Mitogen-activated Protein Kinase Kinase 1
Alternate Names
MAP2K1, MKK1
Gene Symbol
MAP2K1
Additional MEK1 Products
Product Documents for MEK1 Antibody
Product Specific Notices for MEK1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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