MEK2 Antibody (6D5K1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15651

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Applications

Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6D5K1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MEK2 Antibodies »

Product Summary for MEK2 Antibody (6D5K1)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MEK2 (P36507). MLARRKPVLPALTINPTIAEGPSPTSEGASEANLVDLQKKLEELELDEQQKKRLEAFLTQKAKVGELKDDDFERISELGAGNGGVVTKVQHRPSGLIMAR

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MEK2 Antibody (6D5K1)

Western Blot: MEK2 Antibody (6D5K1) [NBP3-15651]

Western Blot: MEK2 Antibody (6D5K1) [NBP3-15651]

Western Blot: MEK2 Antibody (6D5K1) [NBP3-15651] - Western blot analysis of extracts of various cell lines, using MEK2 antibody (NBP3-15651) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Knockout Validated: MEK2 Antibody (6D5K1) [NBP3-15651]

Knockout Validated: MEK2 Antibody (6D5K1) [NBP3-15651]

Knockout Validated: MEK2 Antibody (6D5K1) [NBP3-15651] - Western blot analysis of extracts from normal (control) and MEK2 knockout (KO) HeLa cells, using MEK2 antibody (NBP3-15651) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Applications for MEK2 Antibody (6D5K1)

Application
Recommended Usage

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MEK2

MEK1 and MEK2 (MAPK kinase 1/2, or ERK kinase 1/2) are mitogen-activated protein kinases that stimulate MAP kinase activity, playing a role in both cell growth and differentiation (1,2). MEK itself is activated via phosphorylation at serines 217/218 and 221/222 by upstream activator kinases, including c-raf, mos and MEK kinase (3,4). MEK1 and MEK2 are activated by a wide variety of growth factors and cytokines, and also by membrane depolarization and calcium influx (5).

Long Name

Mitogen-activated Protein Kinase Kinase 2

Alternate Names

MAP2K2, MKK2

Gene Symbol

MAP2K2

Additional MEK2 Products

Product Documents for MEK2 Antibody (6D5K1)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MEK2 Antibody (6D5K1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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