MEK2 Antibody (OTI8G6) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-72646

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI8G6

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all MEK2 Antibodies »

Product Specifications

Immunogen

Full-length protein expressed in 293T cell transfected with human MAP2K2 expression vector

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

44.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MEK2 Antibody (OTI8G6) - Azide and BSA Free

Western Blot: MEK2 Antibody (OTI8G6)Azide and BSA Free [NBP2-72646]

Western Blot: MEK2 Antibody (OTI8G6)Azide and BSA Free [NBP2-72646]

Western Blot: MEK2 Antibody (OTI8G6) - Azide and BSA Free [NBP2-72646] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MEK2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MEK2.
Immunohistochemistry: MEK2 Antibody (OTI8G6) - Azide and BSA Free [NBP2-72646]

Immunohistochemistry: MEK2 Antibody (OTI8G6) - Azide and BSA Free [NBP2-72646]

Immunohistochemistry: MEK2 Antibody (OTI8G6) - Azide and BSA Free [NBP2-72646] - Staining of paraffin-embedded Human Kidney tissue using anti-MEK2 mouse monoclonal antibody.
Flow Cytometry: MEK2 Antibody (OTI8G6) - Azide and BSA Free [NBP2-72646]

Flow Cytometry: MEK2 Antibody (OTI8G6) - Azide and BSA Free [NBP2-72646]

Flow Cytometry: MEK2 Antibody (OTI8G6) - Azide and BSA Free [NBP2-72646] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-MEK2 antibody, and then analyzed by flow cytometry.
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Applications for MEK2 Antibody (OTI8G6) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MEK2

MEK1 and MEK2 (MAPK kinase 1/2, or ERK kinase 1/2) are mitogen-activated protein kinases that stimulate MAP kinase activity, playing a role in both cell growth and differentiation (1,2). MEK itself is activated via phosphorylation at serines 217/218 and 221/222 by upstream activator kinases, including c-raf, mos and MEK kinase (3,4). MEK1 and MEK2 are activated by a wide variety of growth factors and cytokines, and also by membrane depolarization and calcium influx (5).

Long Name

Mitogen-activated Protein Kinase Kinase 2

Alternate Names

MAP2K2, MKK2

Gene Symbol

MAP2K2

Additional MEK2 Products

Product Documents for MEK2 Antibody (OTI8G6) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MEK2 Antibody (OTI8G6) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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