MEK2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38660

Novus Biologicals, part of Bio-Techne
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NBP2-38660
NBP2-38660-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MEK2 Antibodies »

Product Summary for MEK2 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: MLARRKPVLPALTINPTIAEGPSPTSEGASEANLVDLQKKLEEL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MEK2 Antibody

Western Blot: MEK2 Antibody [NBP2-38660]

Western Blot: MEK2 Antibody [NBP2-38660]

Western Blot: MEK2 Antibody [NBP2-38660] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG
Immunocytochemistry/ Immunofluorescence: MEK2 Antibody [NBP2-38660]

Immunocytochemistry/ Immunofluorescence: MEK2 Antibody [NBP2-38660]

Immunocytochemistry/Immunofluorescence: MEK2 Antibody [NBP2-38660] - Immunofluorescent staining of human cell line MCF7 shows localization to cytosol.
Immunohistochemistry: MEK2 Antibody [NBP2-38660]

Immunohistochemistry: MEK2 Antibody [NBP2-38660]

Immunohistochemistry: MEK2 Antibody [NBP2-38660] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.

Applications for MEK2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MEK2

MEK1 and MEK2 (MAPK kinase 1/2, or ERK kinase 1/2) are mitogen-activated protein kinases that stimulate MAP kinase activity, playing a role in both cell growth and differentiation (1,2). MEK itself is activated via phosphorylation at serines 217/218 and 221/222 by upstream activator kinases, including c-raf, mos and MEK kinase (3,4). MEK1 and MEK2 are activated by a wide variety of growth factors and cytokines, and also by membrane depolarization and calcium influx (5).

Long Name

Mitogen-activated Protein Kinase Kinase 2

Alternate Names

MAP2K2, MKK2

Gene Symbol

MAP2K2

UniProt

P36507

Additional MEK2 Products

Product Documents for MEK2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MEK2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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