MEK5 Antibody (3E10)

Novus Biologicals, part of Bio-Techne | Catalog # H00005607-M11

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3E10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MEK5 Antibodies »

Product Specifications

Immunogen

MAP2K5 (AAH08838, 1 a.a. ~ 120 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MLWLALGPFPAMENQVLVIRIKIPNSGAVDWTVHSGPQLLFRDVLDVIGQVLPEATTTAFEYEDEDGDRITVRSDEEMKAMLSYYYSTVMEQQVNGQLIEPLQIFPRACKPPGERNIHGL

Specificity

MAP2K5 - mitogen-activated protein kinase kinase 5

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for MEK5 Antibody (3E10)

Western Blot: MEK5 Antibody (3E10) [H00005607-M11]

Western Blot: MEK5 Antibody (3E10) [H00005607-M11]

Western Blot: MEK5 Antibody (3E10) [H00005607-M11] - Analysis of MAP2K5 expression in Jurkat (Cat # L017V1).
Immunocytochemistry/ Immunofluorescence: MEK5 Antibody (3E10) [H00005607-M11]

Immunocytochemistry/ Immunofluorescence: MEK5 Antibody (3E10) [H00005607-M11]

Immunocytochemistry/Immunofluorescence: MEK5 Antibody (3E10) [H00005607-M11] - Analysis of monoclonal antibody to MAP2K5 on HeLa cell. Antibody concentration 10 ug/ml
ELISA: MEK5 Antibody (3E10) [H00005607-M11]

ELISA: MEK5 Antibody (3E10) [H00005607-M11]

ELISA: MEK5 Antibody (3E10) [H00005607-M11] - Detection limit for recombinant GST tagged MAP2K5 is approximately 0.1ng/ml as a capture antibody.

Applications for MEK5 Antibody (3E10)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MEK5

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been described.

Long Name

Mitogen-activated Protein Kinase Kinase 5

Alternate Names

MAP2K5, MAPKK5, MKK5, PRKMK5

Entrez Gene IDs

5607 (Human)

Gene Symbol

MAP2K5

OMIM

602520 (Human)

UniProt

AAH08838 (Human)

Additional MEK5 Products

Product Documents for MEK5 Antibody (3E10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MEK5 Antibody (3E10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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