MEK5 [p Ser311, p Thr315] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-61105

Novus Biologicals, part of Bio-Techne
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NB100-61105
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Key Product Details

Species Reactivity

Validated:

Rat

Predicted:

Bovine (100%), Canine (100%), Chicken (100%), Human (100%), Mouse (100%), Primate (100%), Xenopus (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MEK5 Antibodies »

Product Summary for MEK5 [p Ser311, p Thr315] Antibody

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser311/Thr315 conjugated to KLH. Accession # Q62862

Modification

p Ser311, p Thr315

Specificity

Specific for endogenous levels of the ~49 kDa MEK5 protein phosphorylated at Ser311, Thr315. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MEK5 [p Ser311, p Thr315] Antibody

Western Blot: MEK5 [p Ser311, p Thr315] Antibody [NB100-61105]

Western Blot: MEK5 [p Ser311, p Thr315] Antibody [NB100-61105]

Western Blot: MEK5 [p Ser311, p Thr315] Antibody [NB100-61105] - Rat testes lysate showing specific labeling of the ~49k MEK5 protein (Control). Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide) but not by the corresponding dephospho-peptide (not shown).

Applications for MEK5 [p Ser311, p Thr315] Antibody

Application
Recommended Usage

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MEK5

MEK5 (also known as MKK5) is a dual specificity serine/threonine protein kinase belonging to the MAP kinase kinase family. MEK5 has been shown to specifically activate ERK5 (Zhou et al., 1995) whereas MEK5 itself is regulated by MEKK3 (Chao et al., 1999). An important link between MEK5 and metastatic prostrate cancer has been demonstrated (Mehta et.al., 2003). Dual phosphorylation of Ser311 and Thr315 have been implicated in cell proliferation (Cameron et al., 2004).

Long Name

Mitogen-activated Protein Kinase Kinase 5

Alternate Names

MAP2K5, MAPKK5, MKK5, PRKMK5

Entrez Gene IDs

29568 (Rat)

Gene Symbol

MAP2K5

UniProt

Q62862 (Rat)

Additional MEK5 Products

Product Documents for MEK5 [p Ser311, p Thr315] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MEK5 [p Ser311, p Thr315] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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