MEKK2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48554

Novus Biologicals, part of Bio-Techne
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NBP2-48554
NBP2-48554-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (91%), Rat (91%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MEKK2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: IAFGQSMDLHYTNNELVIPLTTQDDLDKAVELLDRSIHMKSLKILLVINGSTQATNLEPLPSLEDLDNTVFGAERKKRLSIIGPTSRDRSSPPPGYIPDELHQVARNGSFTSINSEGEFIPESMDQM

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MEKK2 Antibody

Western Blot: MEKK2 Antibody [NBP2-48554]

Western Blot: MEKK2 Antibody [NBP2-48554]

Western Blot: MEKK2 Antibody [NBP2-48554] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: Human cell line RT-4
Immunocytochemistry/ Immunofluorescence: MEKK2 Antibody [NBP2-48554]

Immunocytochemistry/ Immunofluorescence: MEKK2 Antibody [NBP2-48554]

Immunocytochemistry/Immunofluorescence: MEKK2 Antibody [NBP2-48554] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: MEKK2 Antibody [NBP2-48554]

Immunohistochemistry-Paraffin: MEKK2 Antibody [NBP2-48554]

Immunohistochemistry-Paraffin: MEKK2 Antibody [NBP2-48554] - Staining of human gallbladder shows moderate cytoplasmic positivity in glandular cells.

Applications for MEKK2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MEKK2

MEKK2 is a Ser/Thr protein kinase belonging to the MEKK/STE11 subgroup of the MAP/ERK kinase kinase family (1). Closely related to MEKK3, MEKK2 is expressed in a multitude of tissues. MEKK2 directly phosphorylates and activates Ikappa B kinases and has been found to bind and activate protein kinase C-related kinase 2 (2). MEKK2 has also been known to activate JNK1/2, ERK1/2, p38, and ERK5 MAPK in vitro (3). MEKK2 interacting protein (Mip1) regulates MEKK2 expression (4).

Long Name

Mitogen-activated Protein Kinase Kinase Kinase 2

Alternate Names

MAP3K2, MAPKKK2

Gene Symbol

MAP3K2

Additional MEKK2 Products

Product Documents for MEKK2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MEKK2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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