MEKK3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-17273

Novus Biologicals, part of Bio-Techne
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NBP2-17273
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (97%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MEKK3 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human MEKK3. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

71 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MEKK3 Antibody

Western Blot: MEKK3 Antibody [NBP2-17273]

Western Blot: MEKK3 Antibody [NBP2-17273]

Western Blot: MEKK3 Antibody [NBP2-17273] - A. 30 ug 293T whole cell extract B. 30 ug whole cell extract of 3xFlag-human MAP3K3-transfected 293T cells 7.5 % SDS-PAGE MEKK3 antibody [N3C2], Internal dilution: 1:5000
Immunohistochemistry-Paraffin: MEKK3 Antibody [NBP2-17273]

Immunohistochemistry-Paraffin: MEKK3 Antibody [NBP2-17273]

Immunohistochemistry-Paraffin: MEKK3 Antibody [NBP2-17273] - Immunohistochemical analysis of paraffin-embedded Gastric ca, using antibody at 1:500 dilution.

Applications for MEKK3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MEKK3

This gene product is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]

Long Name

Mitogen-activated Protein Kinase Kinase Kinase 3

Alternate Names

MAP3K3, MAPKKK3

Gene Symbol

MAP3K3

UniProt

Q99759 (Human)

Additional MEKK3 Products

Product Documents for MEKK3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MEKK3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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