Melan-A/MART-1 Antibody (A103 + M2-7C10 + M2-9E3)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34244
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A103 + M2-7C10 + M2-9E3) [NBP2-34244]](https://resources.bio-techne.com/images/products/Melan-A-MART-1-Antibody-A103-+-M2-7C10-+-M2-9E3-Immunohistochemistry-Paraffin-NBP2-34244-img0006.jpg "Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A103 + M2-7C10 + M2-9E3) [NBP2-34244]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Canine
Cited:
Human
Applications
Validated:
Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Kappa/IgG2b Lambda/IgG2b Kappa Clone # A103 + M2-7C10 + M2-9E3
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant full-length hMelan-A/MART-1 protein (A103; M2-7C10; M2-9E3) (Uniprot: Q16655)
Localization
Cytoplasmic
Specificity
This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This monoclonal antibody cocktail labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
Marker
Melanoma Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 Kappa/IgG2b Lambda/IgG2b Kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34546)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Melan-A/MART-1 Antibody (A103 + M2-7C10 + M2-9E3)
Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A103 + M2-7C10 + M2-9E3) [NBP2-34244]
Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A103 + M2-7C10 + M2-9E3) [NBP2-34244] - Paraffin-embedded human melanoma. pH 9 antigen retrieval. Image from verified customer review. Image using the Azide and BSA Free format of this antibody.
Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A103 + M2-7C10 + M2-9E3) [NBP2-34244]
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A103 + M2-7C10 + M2-9E3) [NBP2-34244]](https://resources.bio-techne.com/images/products/Melan-A-MART-1-Antibody-A103-+-M2-7C10-+-M2-9E3-Immunohistochemistry-Paraffin-NBP2-34244-img0001.jpg "Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A103 + M2-7C10 + M2-9E3) [NBP2-34244]")
Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A103 + M2-7C10 + M2-9E3) [NBP2-34244]
Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A103 + M2-7C10 + M2-9E3) [NBP2-34244] - Formalin-fixed, paraffin-embedded human melanoma stained withMelan-A MAb (A103+M2-7C10+M2-9E3). Note cytoplasmic staining of cells.Applications for Melan-A/MART-1 Antibody (A103 + M2-7C10 + M2-9E3)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Melan-A/MART-1
Alternate Names
Antigen LB39-AA, Antigen SK29-AA, MART-1, MelanA, MLANA
Gene Symbol
MLANA
UniProt
Additional Melan-A/MART-1 Products
Product Documents for Melan-A/MART-1 Antibody (A103 + M2-7C10 + M2-9E3)
Product Specific Notices for Melan-A/MART-1 Antibody (A103 + M2-7C10 + M2-9E3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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