Melan-A/MART-1 Antibody (CL12863)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18576

Novus Biologicals, part of Bio-Techne
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NBP3-18576-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL12863

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Melan-A/MART-1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein of Q16655. Actual immunogen sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Melan-A/MART-1 Antibody (CL12863)

Western Blot: Melan-A/MART-1 Antibody (CL12863) [NBP3-18576]

Western Blot: Melan-A/MART-1 Antibody (CL12863) [NBP3-18576]

Western Blot: Melan-A/MART-1 Antibody (CL12863) [NBP3-18576] - Analysis in human cell line SK-MEL-30 and human cell line U-2 OS.
Immunocytochemistry/ Immunofluorescence: Melan-A/MART-1 Antibody (CL12863) [NBP3-18576]

Immunocytochemistry/ Immunofluorescence: Melan-A/MART-1 Antibody (CL12863) [NBP3-18576]

Immunocytochemistry/Immunofluorescence: Melan-A/MART-1 Antibody (CL12863) [NBP3-18576] - Staining of SK-MEL-30 cells using the Anti-MART1 monoclonal antibody, showing specific staining in endoplasmic reticulum in green. Nuclear probes are visualized in blue (where available).
Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (CL12863) [NBP3-18576]

Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (CL12863) [NBP3-18576]

Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (CL12863) [NBP3-18576] - Staining of human prostate shows no positivity in glandular cells as expected.
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Applications for Melan-A/MART-1 Antibody (CL12863)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For use with IHC-Paraffin, HIER pH 6 retrieval is recommended. For use with ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Melan-A/MART-1

MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum.

Alternate Names

Antigen LB39-AA, Antigen SK29-AA, MART-1, MelanA, MLANA

Gene Symbol

MLANA

Additional Melan-A/MART-1 Products

Product Documents for Melan-A/MART-1 Antibody (CL12863)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Melan-A/MART-1 Antibody (CL12863)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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