Melan-A/MART-1 Antibody (M2-7C10 + M2-9E3)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34245
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-7C10 + M2-9E3) [NBP2-34245]](https://resources.bio-techne.com/images/products/Melan-A-MART-1-Antibody-M2-7C10-+-M2-9E3-Immunohistochemistry-Paraffin-NBP2-34245-img0001.jpg "Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-7C10 + M2-9E3) [NBP2-34245]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse
Applications
Validated:
Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Lambda/IgG2b Kappa Clone # M2-7C10 + M2-9E3
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant full-length hMelan-A/MART-1 protein (M2-7C10; M2-9E3) (Uniprot: Q16655)
Reactivity Notes
Use in Mouse reported in scientific literature (PMID:33857287)
Localization
Cytoplasmic
Specificity
This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This monoclonal antibody labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
Marker
Melanoma Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Lambda/IgG2b Kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34680)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Melan-A/MART-1 Antibody (M2-7C10 + M2-9E3)
Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-7C10 + M2-9E3) [NBP2-34245]
Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-7C10 + M2-9E3) [NBP2-34245] - Formalin-fixed, paraffin-embedded human melanoma stained withMelan-A MAb (M2-7C10+M2-9E3). Note cytoplasmic staining of cells.Applications for Melan-A/MART-1 Antibody (M2-7C10 + M2-9E3)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml /ml
Western Blot
1-2 ug/ml
Application Notes
Use in Immunohistochemistry reported in scientific literature (PMID:33857287)
Immunohistochemistry (Formalin-fixed): 1-2ug/ml /ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml /ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Melan-A/MART-1
Alternate Names
Antigen LB39-AA, Antigen SK29-AA, MART-1, MelanA, MLANA
Gene Symbol
MLANA
UniProt
Additional Melan-A/MART-1 Products
Product Documents for Melan-A/MART-1 Antibody (M2-7C10 + M2-9E3)
Product Specific Notices for Melan-A/MART-1 Antibody (M2-7C10 + M2-9E3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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