Melan-A/MART-1 Antibody (M2-9E3) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33150
![Western Blot: Melan-A/MART-1 Antibody (M2-9E3)Azide and BSA Free [NBP2-33150]](https://resources.bio-techne.com/images/products/Melan-A-MART-1-Antibody-M2-9E3-Azide-and-BSA-Free-Western-Blot-NBP2-33150-img0003.jpg "Western Blot: Melan-A/MART-1 Antibody (M2-9E3)Azide and BSA Free [NBP2-33150]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # M2-9E3
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Summary for Melan-A/MART-1 Antibody (M2-9E3) - Azide and BSA Free
Immunogen
Recombinant Melan-A/MART-1 protein (Uniprot: Q16655)
Localization
Cytoplasmic
Specificity
This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This monoclonal antibody labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
Marker
Melanoma Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-15198).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Melan-A/MART-1 Antibody (M2-9E3) - Azide and BSA Free
Western Blot: Melan-A/MART-1 Antibody (M2-9E3)Azide and BSA Free [NBP2-33150]
Western Blot: Melan-A/MART-1 Antibody (M2-9E3) - Azide and BSA Free [NBP2-33150] - Western Blot Analysis of COLO38 cell lysate using Melan-A/MART-1 Antibody (M2-9E3).![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-9E3) - Azide and BSA Free [NBP2-33150]](https://resources.bio-techne.com/images/products/Melan-A-MART-1-Antibody-M2-9E3-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-33150-img0002.jpg "Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-9E3) - Azide and BSA Free [NBP2-33150]")
Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-9E3) - Azide and BSA Free [NBP2-33150]
Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-9E3) - Azide and BSA Free [NBP2-33150] - Formalin-paraffin human melanoma stained with Melan-A Ab (M2-9E3). Note cytoplasmic staining of cells.![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-9E3) - Azide and BSA Free [NBP2-33150]](https://resources.bio-techne.com/images/products/Melan-A-MART-1-Antibody-M2-9E3-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-33150-img0001.jpg "Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-9E3) - Azide and BSA Free [NBP2-33150]")
Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-9E3) - Azide and BSA Free [NBP2-33150]
Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-9E3) - Azide and BSA Free [NBP2-33150] - Formalin-paraffin human melanoma stained with Melan-A Ab (M2-9E3). Note cytoplasmic staining of cells.Applications for Melan-A/MART-1 Antibody (M2-9E3) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.5-1ug/ml
Immunohistochemistry-Paraffin
0.5-1ug/ml
Western Blot
0.5-1.0ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Melan-A/MART-1
Alternate Names
Antigen LB39-AA, Antigen SK29-AA, MART-1, MelanA, MLANA
Gene Symbol
MLANA
Additional Melan-A/MART-1 Products
Product Documents for Melan-A/MART-1 Antibody (M2-9E3) - Azide and BSA Free
Product Specific Notices for Melan-A/MART-1 Antibody (M2-9E3) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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