Melanotransferrin/CD228/MFI2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85777

Novus Biologicals, part of Bio-Techne
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NBP1-85777-25ul
NBP1-85777
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Melanotransferrin/CD228 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RSEDYELLCPNGARAEVSQFAACNLAQIPPHAVMVRPDTNIFTVYGLLDKAQDLFGDDHNKNGFKMFDSSNYHGQDLLFKDATVRAVPVGEKTTYRGWLGLDYVAALEGMSSQQC

Reactivity Notes

Mouse (87%), Rat (86%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Melanotransferrin/CD228/MFI2 Antibody

Immunohistochemistry-Paraffin: Melanotransferrin/CD228/MFI2 Antibody [NBP1-85777]

Immunohistochemistry-Paraffin: Melanotransferrin/CD228/MFI2 Antibody [NBP1-85777]

Immunohistochemistry-Paraffin: Melanotransferrin/CD228/MFI2 Antibody [NBP1-85777] - Analysis in human salivary gland and skeletal muscle tissues. Corresponding MELTF RNA-seq data are presented for the same tissues.
Western Blot: Melanotransferrin/CD228/MFI2 Antibody [NBP1-85777]

Western Blot: Melanotransferrin/CD228/MFI2 Antibody [NBP1-85777]

Western Blot: Melanotransferrin/CD228/MFI2 Antibody [NBP1-85777] - Analysis in human cell lines SK-MEL-30 and HeLa. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HDAC1.
Immunohistochemistry-Paraffin: Melanotransferrin/CD228/MFI2 Antibody [NBP1-85777]

Immunohistochemistry-Paraffin: Melanotransferrin/CD228/MFI2 Antibody [NBP1-85777]

Immunohistochemistry-Paraffin: Melanotransferrin/CD228/MFI2 Antibody [NBP1-85777] - Staining of human malignant melanoma shows moderate membranous positivity in tumor cells.
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Applications for Melanotransferrin/CD228/MFI2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Melanotransferrin/CD228

MFI2 is encoded by this gene is a cell-surface glycoprotein found on melanoma cells. The protein shares sequence similarity and iron-binding properties with members of the transferrin superfamily. The importance of the iron binding function has not yet been identified. This gene resides in the same region of chromosome 3 as members of the transferrin superfamily. Alternative splicing results in two transcript variants.

Alternate Names

CD228, MAP97, MFI2, MTF, p97

Gene Symbol

MELTF

Additional Melanotransferrin/CD228 Products

Product Documents for Melanotransferrin/CD228/MFI2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Melanotransferrin/CD228/MFI2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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