MELK Antibody (4F10X4)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16211
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 4F10X4
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for MELK Antibody (4F10X4)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MELK (NP_055606.1). MKDYDELLKYYELHETIGTGGFAKVKLACHILTGEMVAIKIMDKNTLGSDLPRIKTEIEALKNLRHQHICQLYHVLETANKIFMVLEYCPGGELFDYIIS
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for MELK Antibody (4F10X4)
![Western Blot: MELK Antibody (4F10X4) [NBP3-16211]](https://resources.bio-techne.com/images/products/MELK-Antibody-4F10X4-Western-Blot-NBP3-16211-img0001.jpg "Western Blot: MELK Antibody (4F10X4) [NBP3-16211]")
Western Blot: MELK Antibody (4F10X4) [NBP3-16211]
Western Blot: MELK Antibody (4F10X4) [NBP3-16211] - Western blot analysis of extracts of various cell lines, using MELK Rabbit mAb (NBP3-16211) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.Applications for MELK Antibody (4F10X4)
Application
Recommended Usage
Western Blot
1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: MELK
Long Name
Maternal Embryonic Leucine Zipper Kinase
Alternate Names
PK38
Gene Symbol
MELK
Additional MELK Products
Product Documents for MELK Antibody (4F10X4)
Product Specific Notices for MELK Antibody (4F10X4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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