MELK Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83031

Novus Biologicals, part of Bio-Techne
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NBP1-83031
NBP1-83031-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MELK Antibodies »

Product Summary for MELK Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DIKSNNWSLEDVTASDKNYVAGLIDYDWCEDDLSTGAATPRTSQFTKYWTESNGVESKSLTPALCRTPANKLKNKENVYTPKSAVKNEEYFMFPEPKTPVNKNQHKREILTTPNRYTTPSKARNQCLKETPIKIPVNSTGTD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MELK Antibody

Western Blot: MELK Antibody [NBP1-83031]

Western Blot: MELK Antibody [NBP1-83031]

Western Blot: MELK Antibody [NBP1-83031] - Analysis in control (vector only transfected HEK293T lysate) and MELK over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: MELK Antibody [NBP1-83031]

Immunohistochemistry-Paraffin: MELK Antibody [NBP1-83031]

Immunohistochemistry-Paraffin: MELK Antibody [NBP1-83031] - Staining of human stomach shows strong cytoplasmic and nuclear positivity in glandular cells.

Applications for MELK Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MELK

MELK is a member of AMPK family, which has several consensus glycosylation sites. Glycosylations are known to affect migration of proteins on a SDS-PAGE [Hames, B.D and Rickwood, D., eds. Gel electrophoresis of proteins: a practical approach. Oxford University Press]. Further the activity of this antibody was confirmed against recombinant, GST fused MELK protein by Western blotting.

Long Name

Maternal Embryonic Leucine Zipper Kinase

Alternate Names

PK38

Gene Symbol

MELK

Additional MELK Products

Product Documents for MELK Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MELK Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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